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Vitamin B1 biosynthesis in plants requires the essential iron–sulfur cluster protein, THIC ==================================================================== 1. Maja Raschke,
2. Lukas Bürkle, 3. Nadine Müller,
4. Adriano Nunes-Nesi†, 5. Alisdair R. Fernie†,
6. Duilio Arigoni‡, 7. Nikolaus Amrhein, and
8. Teresa B. Fitzpatrick,§ 1.
ETH Zurich, Institute of Plant Sciences, 8092 Zurich, Switzerland; 2.
†Max-Planck-Institute for Molecular Plant Physiology, 14476 Potsdam-Golm, Germany; and 3.
‡ETH Zurich, Organic Chemistry Laboratory, 8093 Zurich, Switzerland 1.
Communicated by Roland Douce, Université de Grenoble, Grenoble, France, October 12, 2007 (received for review September 4, 2007) Abstract --------
Vitamin B1 (thiamin) is an essential compound in all organisms acting as a cofactor in key metabolic reactions and has furthermore been implicated in responses to DNA damage and pathogen attack in plants. Despite the fact that it was discovered almost a century ago and deficiency is a widespread health problem, much remains to be deciphered about its biosynthesis. The vitamin is composed of a thiazole and pyrimidine heterocycle, which can be synthesized by prokaryotes, fungi, and plants. Plants are the major source of the vitamin in the human diet, yet little is known about the biosynthesis of the compound therein. In particular, it has never been verified whether the pyrimidine heterocycle is derived from purine biosynthesis through the action of the THIC protein as in bacteria, rather than vitamin B6 and histidine as demonstrated for fungi. Here, we identify a homolog of THIC in Arabidopsis and demonstrate its essentiality not only for vitamin B1 biosynthesis, but also plant viability. This step takes place in the chloroplast and appears to be regulated at several levels, including through the presence of a riboswitch in the 3′-untranslated region of THIC. Strong evidence is provided for the involvement of an iron–sulfur cluster in the remarkable chemical rearrangement reaction catalyzed by the THIC protein for which there is no chemical precedent. The results suggest that vitamin B1 biosynthesis in plants is in fact more similar to prokaryotic counterparts and that the THIC protein is likely to be the key regulatory protein in the pathway. metabolites
plant viability riboswitch
thiamin Arabidopsis
Footnotes
---------
§To whom correspondence should be addressed. E-mail:
tfitzpatrick{at}ethz.chAuthor contributions: T.B.F. designed research; M.R., L.B., N.M., A.N.-N., A.R.F., and T.B.F. performed research; M.R. and T.B.F. contributed new reagents/analytic tools; M.R., L.B., N.M., A.N.-N., A.R.F., D.A., N.A., and T.B.F. analyzed data; and T.B.F. wrote the paper.
The authors declare no conflict of interest.
This article contains supporting information online at www.pnas.org/cgi/content/full/0709597104/DC1.
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This Article 1. Published online before print November 28, 2007, doi: 10.1073/pnas.0709597104 PNAS December 4, 2007 vol. 104 no. 49 19637-19642
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